细菌培养与涂片镜检在微生物检验中的价值对比

Objective To evaluate the application value of bacterial culture and smear microscopic examination in microbiological testing. Methods A total of 154 microbial samples from 2023 to 2025 were included and all underwent bacterial culture and smear microscopic examination. The screening pass rates of the two testing methods were compared. Results There was no statistically significant difference in the detection rates of salmonella, staphylococcus aureus, bacillus cereus, listeria monocytogenes and diarrheagenic escherichia coli between the two testing methods(P>0.05). Conclusion During the microbial testing process, microscopic examination of the smears is of great significance for the bacterial culture operation. This step can determine whether there are bacteria, fungi and other microorganisms in the sample, preliminarily identify their morphological characteristics and estimate their quantity, thereby reducing errors caused by sample quality or operational mistakes. Moreover, it also helps to assess the compliance and representativeness of sample collection, ensuring that the submitted samples meet the requirements of subsequent culture and improving the accuracy and reliability of the test results.