反相高效液相色谱混标加样增量法快速测定食品中的阿斯巴甜和阿力甜

Objective To analyze the value of reversed-phase high performance liquid chromatography(RP-HPLC) mixed standard and incremental method for rapid determination of aspartame and alitame in food. Methods Test samples: 80 food samples, including pastries, candies, and beverages. Sample processing: Ultrasonic extraction, centrifugal purification, quantitative analysis using mixed standard and incremental method, constructing calibration curves based on a series of concentration mixed standard solutions, eliminating matrix interference using incremental addition, and shortening pretreatment time. System validation includes linear range, detection limit(LOD), quantification limit(LOQ), precision, accuracy, and matrix effects. Results The recovery rate of the mixed standard incremental method is 93.6% to 102.1%, indicating a significant elimination effect of matrix effects. Under the standard range of 0.05～5.00 mg/mL for aspartame and 0.02～2.00 mg/mL for alitame, the linear relationship is good and meets the requirements of quantitative analysis. At a signal-to-noise ratio of 10 times(S/N=10), the LOD and LOQ of aspartame and alitame are both lower than the maximum usage limit specified in China's 2760-2014, which meets the requirements for detecting trace sweeteners in food. The recovery rates of each sample spiked were all over 90%, with relative standard deviation(RSD) ranging from 1.5% to 3.8%, which meets the accuracy requirements of trace analysis methods. The content range of aspartame and alitame is within the standard range, and there are no samples exceeding the standard. Conclusion The RP-HPLC mixed standard incremental method is easy to operate and requires a short time, which can meet the needs of rapid batch detection of aspartame and alitame in food, and has high application value.