去抗原马鹿角粉调控巨噬细胞M2极化促进颌骨缺损再生的机制研究

Objective To explore the mechanism by which the antigen-removed an antigen-removed calcined antler cancellous bone powder(CACB) regulates macrophage M2 polarization and promotes bone regeneration in jaw defects. Methods The CACB extract solution was prepared from the characteristic horse antler of Xinjiang. A jaw defect model was established in SD rats. The high and low concentrations of CACB extract solution and scaffold materials were combined and re-implanted into the rats, and the bone formation, inflammatory response-related indicators, and biomechanical properties were detected at 4, 8, and 12 weeks after implantation. Results The BMD and BV/TV of the high-concentration CACB group were higher than those of the control group at 4, 8, and 12 weeks after surgery(P<0.05). The levels of IL-1β and TNF-α in the high-concentration CACB group were lower than those in the control group at 4 and 8 weeks after surgery, while the level of IL-10 was higher than that in other group(P<0.05). The mRNA expression levels of ALP and OCN in the high-concentration CACB group were higher than those in the control group at 4 and 8 weeks after surgery; the compressive strength, elastic modulus, and maximum load of the high-concentration CACB group at 12 weeks were higher than those of the other groups(P<0.05). Conclusion The antigen-removed antigen-removed calcined antler cancellous bone powder extract solution regulates macrophage M2 polarization, reduces inflammatory response, promotes the expression of bone formation-related factors, effectively promotes jaw defect regeneration.