微小核糖核酸-124调控巨噬细胞成骨分化在种植体周围骨整合中的作用

Objective To explore the regulatory effect of microRNA-124(miR-124) on osteogenic differentiation of bone marrow-derived macrophages and its mechanism in implant osseointegration. Methods Seventy-two SD rats were selected and randomly divided into the control group, the miR-124 overexpression group and the miR-124 inhibition group. The rats were locally transfected with the corresponding vectors after implant surgery. The bone integration rate was evaluated by micro-CT, histological changes were observed by HE staining, the expression of miR-124 was detected by RT-PCR, and the expression of osteogenic markers was analyzed by Western blot. Results The bone integration rate in the miR-124 overexpression group was significantly higher than that in the control group, and that in the miR-124 inhibition group was significantly lower than that in the control group. In the miR-124 overexpression group, new bone formation was active, and the expressions of osteogenic markers were significantly upregulated, while those in the miR-124 inhibition group were significantly downregulated. Conclusion miR-124 promotes osteogenic differentiation of bone marrow-derived macrophages and improves the quality of osseointegration of implants.