富锌硒茶在奥硝唑诱导的弱精症小鼠中的作用与机制

Abstract: Objective To investigate the effect and mechanism of zinc-selenium rich tea on asthenospermia induced by ornidazole in mice. Methods The pathological changes of mouse epididymis were detected by Hematoxylin-Eosin (HE)staining. Real-time PCR was used to detect the gene expression levels of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) in the epididymis of mice. The expression levels of GSH-Px and T-SOD in the testicles of mice were detected by colorimetry and hydroxylamine method. GraphPad Prism 8.0 software was used to analyze the data. Results The result of HE staining showed that compared with the control group, the model group, the zincselenium rich green tea group, and the ordinary green tea group all showed a decrease in sperm in the epididymal cavity. The forward movement of sperm and total sperm motility in the model group were significantly lower than those in the control group. Compared with the model group, the sperm concentration in the zinc-selenium rich green tea group and the ordinary green tea group were significantly increased, and the forward movement of sperm in the zinc-selenium rich green tea was significantly better than that in the ordinary green tea group (P＜0.05). The results of Real-time PCR showed that the messenger ribonucleic acid (mRNA) levels of T-SOD and GSH-Px in the model group were significantly decreased. Compared with the model group, the expression levels in the zinc-selenium rich green tea group and the ordinary green tea group were significantly increased (P＜0.05). The total activities of T-SOD and GSH-Px in the model group were significantly lower than those in the control group, and the activities in the zinc-selenium rich green tea group were between the control group and the model group (P＜0.05). Conclusion Zinc and selenium supplementation may improve the sperm concentration and motility of asthenospermia mice by enhancing the antioxidant capacity of the body, which has certain guiding significance for the treatment and prevention of asthenospermia.