流式细胞仪检测T淋巴细胞亚群在肺部感染性疾病诊断中的价值

Objective To explore the diagnostic value of T lymphocyte subsets detected by flow cytometer in pulmonary infectious diseases. Methods A retrospective analysis was conducted on the clinical data of 100 patients with suspected pulmonary infectious diseases(observation group) admitted to our hospital from February 2023 to March 2025 and 80 healthy individuals undergoing physical examinations during the same period(control group). T lymphocytes were measured by flow cytometer according to the single platform wash free method, and the clinical comprehensive diagnosis was used as the gold standard to evaluate its diagnostic efficacy. Results The combined diagnostic sensitivity and specificity of CD3+ T lymphocytes(CD3~+T cell), CD4+ T lymphocytes(CD4~+T cell), CD4+/CD8+ ratio was 94.67% and 96.00%, respectively AUC was 0.946, the sensitivity was significantly better than single index detection(P<0.05). The level of CD4~+T cell, CD3~+T cell, CD4+/CD8+ ratio in the observation group were lower than those in the control group, and CD8~+T cell level was higher than that in the control group(P<0.05). Conclusion Flow cytometer detection of T lymphocytes subsets provides a reliable tool for immunodiagnosis of pulmonary infectious diseases, and combined detection serves as an important supplement for comprehensive clinical diagnosis to support precise diagnosis and treatment.