微生物检验中微滤膜分离方法的样本污染及检出结果分析

Abstract: Objective To analyze the sample contamination and detection results of the microfiltration membrane separation method in microbiological testing. Methods A total of 122 microbial samples to be tested collected by the Center for Disease Control and Prevention from March 2023 to March 2024 were included in the study. Meanwhile, plate culture method and microfiltration membrane separation technology tests were conducted. The test results between groups and the impact on sample contamination were compared. Results The plate culture method and microfiltration membrane separation technology detected 67 and 64 positive samples from 122 microbial samples to be tested, respectively. There was no statistically significant difference in the initial positive rate, re-positive rate and missed detection rate between the two (P＞0.05). However, the reporting time for microbial testing by microfiltration membrane separation technology was shorter than that of the plate culture method, the sample contamination rate was lower than that of the plate culture method, and the recovery rate of positive bacteria was significantly higher than that of the plate culture method. The difference was statistically significant (P＜0.05). Conclusion The accuracy of microfiltration membrane separation technology in testing microorganisms is basically the same as that of the plate culture method. Moreover, it can significantly shorten the reporting time, reduce sample contamination, and increase the recovery rate of positive bacteria. The overall advantages are more prominent.